In laymens terms, they are siblings born to the same surrogate mother, exhibiting different combinations of the same genetic mixture. A primary function of the core is to provide a centralized laboratory that will generate infection-free strains of mice carrying transgenes or targeted alterations of specific interest to individual projects in the DRC. Free full text J Dent Res. For more information about our services, charges for services or personnel please visit the official Transgenic & Chimeric Mouse Facility. What strain of mice are used as foster mothers? From those 2 males we can freeze down 20 sperm straws (10 straws per male). The tryptophan synthetase gene A series of mutants in E. coli has been used to examine the mutational specificity of over 80 purine base analogues. The Transgenic and Chimeric Mouse Core has generated a wide range of transgenic and gene-targeted mouse lines that have proven to be powerful tools for the study of the molecular biology and physiology of Type I and Type II diabetes, obesity, and developmental and mechanistic bases for a wide variety of other disorders of endocrinology and metabolism. Chemical Libraries: SelleckChem Bioactive Library (2083 cmpds) kinase 373 The tryptophan synthetase gene A series of mutants in E. coli has been used to examine the mutational specificity of over 80 purine base analogues. It also allows for ease of sharing mouse strains with collaborators across the world. Privacy Policy | Penn Vet | Transgenic Mouse Core - University of Pennsylvania School of 1 College Hall, Room 1, These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. They may also result from the crossing of one or more transgenic animals. Philadelphia, PA 19104. The Transgenic and Chimeric Mouse Facility provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. sharing sensitive information, make sure youre on a federal Dr. Karolina Nitsche is the EICF Scientist and Core Director for the Mouse Transgenic and Gene Targeting Core Facility since 2019. . Analysis of the molecular mechanisms involved in chromatin dynamics, homologous recombination, and chromosome segregation during meiosis will elucidate causes for infertility and birth defects as a result of aneuploidy. To achieve these goals, the School of Veterinary Medicine has established the Transgenic Mouse Core. 1 Through intramolecular recombination, cre has the ability to excise loxP-flanked (floxed) sequences from the genome, leaving a single loxP site in its place. Introduction to Anatomy and Physiology II with Lab or Human Anatomy with Lab. Redirecting to /core/journals/journal-of-dairy-research/article/abs/expression-of-bovine-lactoglobulin-transgenic-mice Facility provides centralized service to efficiently produce genetically altered mice for basic research. UPenn users. 8600 Rockville Pike Our primary mission is to undertake innovative research on stem cell biology, germ cell development, and animal transgenesis. The last 4 analogues also cause transitions. Fertilization of mature oocytes with capacitated sperm in a culture dish. Genomic Analysis Core - Abramson Cancer Center | Penn Medicine Steven's animal research career began at University of Pennsylvania as an Animal Laboratory Technician. ES-cell Targeting(129xBl/6-F1 hybrid) ) (up to 96 clones), 3800 Spruce Street - Philadelphia, PA 19104. Mutat Res. Genotoxicity and mutagenicity of Echinodorus macrophyllus (chapu-de-couro) extracts. DMOZ - Science: Biology: Genetics: Eukaryotic: Animal: Mammal: Rodent Kozmin SG, Schaaper RM, Shcherbakova PV, Kulikov VN, Noskov VN, Guetsova ML, Alenin VV, Rogozin IB, Makarova KS, Pavlov YI. Privacy Policy. HighThroughput Screening Core Sara Cherry, Ph. What do I do with my Chimeras after transfer? Associate Dean of Sustainable Agriculture and Veterinary Practices An improved understanding of the basic processes governing germ cell and embryo development and of the biology of gametes (sperm and oocytes) and embryonic stem cells will enable us to improve reproductive efficiency, generate animal models of human and animal disease and help provide the knowledge base for regenerative medicine, as well as toward the treatment of infertility. 3400 Civic Center Boulevard, 12th Floor PDF TRANSGENIC ANIMALS NIH Guidelines for Research Involving - UPenn Federal government websites often end in .gov or .mil. All Rights Reserved. We Recommend males be no younger than 12 weeks before freezing. It is proposed that a major mechanism for hydroxylaminopurine mutagenesis is due to the reaction of these compounds with the O6-position of guanine and the O4-position of thymine. 1985;31:381-9. doi: 10.1007/978-1-4613-2449-2_23. 3400 Civic Center Boulevard, 12th Floor 3800 Spruce Street, OVQ 177E, ULAR, School of Veterinary Medicine, University of Pennsylvania, Philadelphia PA 19104 Phone: 215) 573-3625 (RDS) (215)898-5319 (BHP) Fax . All of the compounds stimulated the AT----GC transition (by up to 1000-fold) and 11 of the 14 base analogues raised the GT----AT transition (by up to 450-fold). Cambridge University Press & Assessment Disclaimer. Personnel. These particular mice were created by our facility for research conducted in the lab of Dr. John R. Stanley, previously Chairman of the Department of Dermatology in the University of Pennsylvania School of Medicine. HHS Vulnerability Disclosure, Help Cores - Penn Genetics 415 Curie Blvd. The Comparative Pathology Core (CPC), an Abramson Cancer Center shared resource, provides expert pathological characterization and validation of mouse and other animal models used in biomedical research by offering the expertise of board-certified veterinary pathologists and access to state-of-the-art histology, molecular staining of animal tissues, and digital pathology services. The center provides a platform for intellectual interactions to facilitate research in reproductive biology across the school and the campus. MeSH Technologies are believed to improve the elderly's daily lives and maintain their health efficiently. Email: nleu@vet.upenn.edu A major objective of this research is to improve the health and productivity of domestic animals by genetic modification and to develop animal models for biomedical research. Transgenic and Chimeric Mouse Facility . About | Emory University | Atlanta GA official website and that any information you provide is encrypted Influence of sex, smoking and age on human hprt mutation frequencies and spectra. TRANSGENIC & GENE TARGETING MOUSE FACILITY Department of Biomedical Sciences School of Veterinary Medicine University of Pennsylvania Phone: 215-573-2302 email: sterlin@vet.upenn.edu Please complete top section Investigator: _____ Department:_____ Other external customers. For more information, please contact Adrian Leu This should result in reduction in effort and cost to participating investigators. The Transgenic Mouse Genome Editing Core has generated a wide range of transgenic and gene-targeted mouse lines that have proven to be powerful tools for the study of the molecular biology and physiology of Type I and Type II diabetes, obesity, and developmental and mechanistic bases for a wide variety of other disorders of endocrinology and metabolism. Penn Vet | Contact Us Contact Us Transgenic Mouse Core Contacts Director: N. Adrian Leu Phone: 215-573-2302 Email : nleu@vet.upenn.edu Transgenic Services, Re-derivation Lab Manager: Stephanie Sterling Phone: 215-573-2301 Email: sterlin@vet.upenn.edu Cryopreservation, Billing, Shipping & Receiving Faculty Oversight: Dr. Jeremy Wang Email: pwang@vet.upenn.edu PDF WHEN - Perelman School of Medicine at the University of Pennsylvania RNA-seq reads were aligned to the . EBF2 promotes the recruitment of beige adipocytes in white adipose However there is a clear indication that such development is shifting towards 1 Volume 1, Number 1, 2006. the peripheries. Office (UPenn) Penn Vector Core Facility (PSOM) Proteomics Core Facility (CHOP) Flow Cytometry Core (CHOP) Molecular Pathology and Imaging Core (PSOM) High - Throughput Screening Core Facility (PSOM) Comparative Medicine Services Core (CHOP) Transgenic Core Facility (CHOP) Center for Advanced Computer Tomography Imaging Services (PSOM) 11:30 a.m. - PDF Biomedical Research Core Facilities - Perelman School of Medicine at Association of Biomolecular Resource Facilities. All 12 hydroxylaminopurine base analogues tested, 2,6-dimethoxyaminopurine and 2-hydrazinopurine were found to cause transition mutations. The animal room provides housing and breeding space for the mice involved in the transgenic projects. Transgenic and Chimeric Mouse Facility - Penn Genetics Major categories . and transmitted securely. The Transgenic and Chimeric Mouse Facility provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. The animal room provides housing and breeding space for the mice involved in the transgenic projects. Despite the advantage of adopting technologies, the elderly are slower to adopt new technologies compared Department of The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, cytoplasmic/pronuclear injections into fertilized oocytes of CRISPR-Cas9 mix (gRNA, Cas9RNA, ssDNA/dsDNA templates), embryo and sperm cryopreservation, in vitro fertilization, and re-derivation of live and cryopreserved lines. Jean Richa, Ph.D. These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. PDF Disposal of Transgenic Arthropods Compliance with the NIH - UPenn We do not charge you a service fee unless we produce a founder. Clipboard, Search History, and several other advanced features are temporarily unavailable. BUMC: Transgenic Mice Core . The facility is located on the basement level of the Clinical Research Building. and re-derivation of live and cryopreserved lines. Unauthorized use of these marks is strictly prohibited. Email: kthompso@pennmedicine.upenn.edu, Tel: 215-573-3023; 215-746-6392 For more information about our services, charges for services or personnel please visit the official Transgenic & Chimeric Mouse Facility. PDF TRANSGENIC & GENE TARGETING MOUSE FACILITY Department of Biomedical For Es Cell derivation & gene targeting please contact Chris Lengner (lengner@vet.upenn.edu) & Stephanie Adams (asteph@vet.upenn.edu). FOIA Adele Harman: harmana@chop.edu: Translational Core. This facility consists of an animal room and several injection rooms. PDF University of Pennsylvania Perelman School of Medicine High Throughput Transgenic Core. Accessibility The Genomic Analysis Core (GAC) of the Abramson Cancer Center (ACC) provides a spectrum of genomic and molecular biological services with a team of highly experienced and trained professionals. an Institute of Gene Therapy, and Transgenic Core Facilities. Memo 3/18/15 regarding PSOM Subvention Requests for Mouse Cryopreservation Services. The Transgenic Mouse Genome Editing Core has generated a wide range of transgenic and gene-targeted mouse lines that have proven to be powerful tools for the study of the molecular biology and physiology of Type I and Type II diabetes, obesity, and developmental and mechanistic bases for a wide variety of other disorders of endocrinology and met. These animals may be purchased through a vendor, received from a collaborator, custom-made by a core or created in your lab. Would you like email updates of new search results? | University of Pennsylvania Perelman School of Medicine High-Throughput Screening Core Sara Cherry, Ph. Care for cats, dogs & other companion animals. The Transgenic Mouse Core at the Center ( Technical director, N. Adrian Leu; Faculty oversight: P. Jeremy Wang MD/PhD and Christopher Lengner, PhD) provides an array of embryological services for the generation and preservation of genetically engineered transgenic and gene knockout mice. D. dschultz@mail.med.upenn.edu (215) 5739641 . Transgenics -- Transgenic Mice - Edinformatics This facility consists of an animal room and several injection rooms. Our liberal arts program helps prepare you for clinical, research, and leadership roles. The primary mission was to undertake innovative research on stem cell biology, germ cell development, and animal transgenesis. Care for cats, dogs & other companion animals. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). 1981 Aug;83(1):25-37. doi: 10.1016/0027-5107(81)90068-3. For sperm cryopreservation we require 2 males per strain. Jean Richa, Ph.D., is the Technical Director in charge of the production of transgenic mice. This should result in reduction in effort and cost to participating investigators. D. David C. Schultz, Ph. The .gov means its official. Douglas Epstein, Ph.D., is the Facility Director. Contact David Schultz at dschultz@mail.med.upenn.edu for an initial consultation Define the project Determine if the facility has relevant expertise/technology to pursue the project 137: 1091-1102, 1997). BCM Mouse Genome Project - Large scale ENU based mouse mutagenesis for developmental defects; BUMC: Transgenic Mice Core - The mouse core facility at Boston University School of Medicine provides centralized, expert service to produce successfully transgenic as well as knock out mice for investigators at Boston University and as an outside service. The Amelogenin C-Terminus Is Required for Enamel Development - ResearchGate Vidal LS, Alves AM, Kuster RM, Lage C, Leito AC. M180 Amelogenin Processed by MMP20 is Sufficient for Decussating Murine Smilow Center for Translational Research 3800 Spruce Street - Philadelphia, PA 19104. All the high throughput sequencing was performed by the Functional Genomics Core at the University of Pennsylvania using HiSeq 2000 or 2500. The amelogenin C-terminus is required for enamel development. - Europe PMC The facility uses sterile food and water as well as autoclaved cages and bedding; all cages are of the microisolator type to limit the spread of colony infection. (J.Cell Biol. The society also sponsors multi-center research studies designed to help members incorporate new biotechnologies into their laboratories. Please enable it to take advantage of the complete set of features! For details, please email the School of Nursing or explore its website. MMP20, a protease secreted by ameloblasts, is responsible for processing enamel proteins, including AMELX, during the secretory stage of enamel formation. The construct was injected into FVB mouse oocytes by the University of Pennsylvania Transgenic and Chimeric Mouse Facility. Nucleic Acids Res. Bethesda, MD 20894, Web Policies Elderly's intention to use technologies: A systematic - PubMed 3800 Spruce Street - Philadelphia, PA 19104, Cas9 protein provided and final injection mixture done in house (unless otherwise specified), Injections done in mixed B6/B6D2 Background, but we also offer pure B6 Background upon request (injection into any other background will incur extra fees), Tail samples will be provided for genotyping by investigators, Desired pups will be transferred at weaning age (21 days), Chimeras will be determinable by coat color, Chimeras will be transferred at weaning age (21 days), The cost of your injection includes 2 clones from one target, We inject an average of 30 embryos per clone, Requesting lab must provided sperm sample, Can be performed using fresh or frozen sperm. 1978 Jan;56(3):225-34. doi: 10.1016/0027-5107(78)90189-6. de Serres FJ, Brockman HE, Hung CY, Overton LK. The School of Nursing is accredited by the Commission on Collegiate Nursing Education (CCNE). Curry J, Karnaoukhova L, Guenette GC, Glickman BW. | Sitemap, Biomedical Research Core Facilities Newsletter, ABRF is a unique membership association comprising over. Academic Services, Office of College Houses and. Gorrod JW, Ioannides C, Lam SP, Neville S. Environ Health Perspect. Cardiac Tissue-Restricted Deletion of Plakoglobin Results in
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